Análisis y cuantificación de Receptores de Estrógenos en la gestación porcina

Abstract

The placenta is a temporaryessential organ for gestation development. Porcine placenta is epitheliochorial, diffuse, adecidua, folded and non-invasive. Reciprocal interactions between the endometrium and the conceptusare required for the maintenance of pregnancy. Synthesized estrogens (Es) by the conceptus promote maternal-fetal recognition during pregnancy. The estrogens effects are mediated by the interaction with their specific nuclear receptors (ER) and G-protein coupled (GPER 30). The aim of this study was to determine the localization of the ERα, ERβand GPER 30 in placental tissues of different gestational periods and in non-pregnant sows. REαand REβexpression was determined by immunohistochemistry in reproductive tracts of pregnant and non-pregnant sows. Estrogen concentration was measured by chemiluminescence. The results were expressed in a qualitative way based on the color detected, determining that (+): nuclear positivity, (-): negative. In order to quantitatively evaluate the expression of estrogen and progesterone receptors at the swine placental interface in the selected gestation periods, images of the immunohistochemistry results were taken for subsequent morphometric and densitometric analysis. The image quantification technique will be used using ImageJ / Fiji software. To analyze the feto-maternal interface from each preparation, images of the endometrial luminal and glandular epithelium will be obtained and the fetal placenta will be extracted from the luminal, endometrial glandular and trophoblastic epithelium. For the morphometric analysis of the area occupied by the molecules of this work, in relation to the total area, both of the endometrial luminal and glandular epithelium and of the trophoblast, the percentage of immunostained area (% AIM) and the optical density (OD) will be determined