Modelo de investigación oncológica para evaluar medicamentos antitumorales

Abstract

In cancer research the use of animal models is controversial because animal well-being is first priority. A canine model is described based on reproductive biopsies without apparent disease. It is also valuable to assess antitumor drug therapies. Morphological changes, variations in the immunohistochemical expression of hormone receptors and levels of cell proliferation in the canine model were analized connected with reproductive biopsies (n= 42) of tissue remains in massive castrations. There were changes associated with hormone blood levels both in treatment an control groups with results related to estrous cycle phases. Exclusion criteria according to the estrous cycle and drug effect were established. Doses previously set in periods were administered. The expression of hormone receptors and proliferation Ki-67 marker were subjected to sampling evaluation. As to the assays, the aglepristone made the progesterone decrease on the seventh day and even more on fourteenth day in the treatment group. Instead, in the control group the descent was minimum.PR expression decreased significantly on day 7 and on day 14 in the treatment group while it increased on day 7 and remained unchanged on day 14. Ki-67 marker showed similar behavior. The cancer model demonstrated the progesterone proliferation role and its suitability for aglepristone reposition as a hormonal post-surgical adjuvant. At present, a testoterone enanthate research with same protocols is carried out.