Variación del MHC-B en Líneas de pollos Campero INTA y en Pollos Parrilleros y su correlación con peso vivo

Abstract

This paper details the research tasks related to the Major Histocompatibility Complex-B (MHC- B) in birds, in which I was able to participate during my experience as a CIN fellowship, under the direction of Mg. Gabriela M. Iglesias. The variability of the MHC-B genes was analyzed through a marker, LEI0258, strongly linked, in maternal (A and ES) and paternal (ES) lines that give rise to the INTA Campero chicken (within the framework of PI 40- A-498 directed by Gabriela Iglesias). In addition, a sample of broilers was genotyped using LEI0258 in order to establish an association between LEI0258 marker and the live weight (LW) of the animals. This group of birds was part of a trial directed by Dra. Belén Buglione, within the framework of PI 40-A-614. As a result, 6 alleles and 18 genotypes were obtained in the different populations that give rise to the Campero INTA, the most variable being the ES line, followed by the A line and the least variable, the AS line. The allele with the highest frequency was that of 381pb. On the other hand, in the broiler chicken trial, the analysis indicated an association trend (p<0.1) between the 357/381 genotype and a higher LW, while the 295/381, with lower LW (it was observed a difference of 460 g). Finally, the model used showed that the *357 allele (frequency 16.7 %) would have a positive association and the 205 allele (frequency 6.7 %) had a negative association (p<0.05) with PV. From the analysis it emerges that the frequency of the alleles with positive association for the PV characteristic (357 and 381 bp) represent approximately 50 % of the alleles in the population studied, so these variants could be positively valued in the development of the selection plan.