Título:	Secondary metabolites of Bougainvillea spinosa with antioxidant activity through callus grown in vitro under physical stress
Autor(es):	Dalzotto, Daniela Cecilia Espíndola, Micaela Huenelaf, Valeria Belén Piñuel, María Lucrecia Sharry, Sandra Elizabeth Boeri, Patricia Alejandra
Fecha de publicación:	17-sep-2021
Revista:	XXXIII Argentinean Meeting of Plant Physiology
Resumen:	Plant tissue culture is an attractive alternative source of bioactive compounds, like secondary metabolites (SM). Elicitation by abiotic factors can be an important strategy towards improved in vitro production of these compounds. Bougainvillea spinosa is a woody species native to Argentina, which has been little studied in this aspect. The objective of this work was to evaluate if different physical stresses can induce the production of SM with antioxidant activity (AO) in B. espinosa callus obtained in vitro. The callus were laid in solid MS medium at half concentration with 30% sucrose for one month in two stress conditions: 1) saline stress with 150 µM NaCl; 2) UV light stress on the fifth day of culture. A control group was maintained in ½ MS. After culture, an extraction of the SM was carried out using 65% methanol as solvent. The AO of the extracts was measured through the DPPH and ABTS methods. The results showed that the stress induced by NaCl failed to elicit the synthesis of SM with AO in respect to the control group. However, extracts obtained from callus exposed to UV light stress increased AO in both methods (91.8±8 and 150.6±7 µmol troloxEQ/100 g fresh callus for DPPH and ABTS, respectively), with respect to the control (72.1±9 and 108.3±4 µmol troloxEQ/100 g fresh callus for DPPH and ABTS, respectively). These results are relevant as they show that UV light stress could be a physical elicitor for obtaining secondary metabolites with antioxidant activity in B. spinosa callus.
URI:	http://rid.unrn.edu.ar/handle/20.500.12049/10287
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